A long oocyte preincubation (9–11 hours) prior to ICSI is thought to have bad effects on embryo quality , probably due to oocyte ageing.However, other studies supported different results as no statistically significant differences in the fertilization [3, 4] or the pregnancy rates [2, 6] were found in preincubated oocytes during ICSI cycles.Semen samples were collected the day of oocyte retrieval by masturbation.After liquefaction, semen was prepared by a 90-45 gradient system using Puresperm (JCD, France).generally_accepted_accounting_practices means a collection of rules and procedures and conventions that define accepted accounting practice; includes broad guidelines as well as detailed procedures; ...To provide a block material to be installed under the sea water that can be constructed over a wide area of the sea that has been already attacked or has the anxiety of being attacked with rocky-shore denudation or the red tide and can manifest the effective preventive effect against rocky-shore denudation or red tide.The purpose of this study was to analyze retrospectively the impact of oocyte denudation and microinjection timings on the ICSI outcome in a selected population so as to carry out corrective measures to improve the results.
Embryos were put into one of 4 categories according to the percentage of anucleate fragments: type A, when there was no anucleate fragmentation, type B when 1–20% of the embryo was fragmented, C when the proportion of fragmentation ranged between 21 and 50% and D when over 50%, of the embryo was fragmented.Therefore preincubation of oocytes prior to IVF or ICSI may induce cytoplasmic maturation that could eventually increase fertilization and also pregnancy rates.Moreover, Balakier and colleagues  reported that human oocytes progressively develop the ability for full activation and normal development during the MII arrest stage.Different timings were analyzed in this retrospective study (Figure 1).The timing between oocyte retrieval and oocytes denudation was the timing elapsed between the beginning of oocyte retrieval and the beginning of oocyte denudation (Assessment of fertilization was made 17-18 h after ICSI by checking the number of polar bodies and pronuclei.The improvement in fertilization rates was obtained when ICSI was carried out 6–8 hours after the first polar body expulsion.For normal fertilization to occur, both nuclear and cytoplasmic maturity is required independently .The microinjection technique has been completely standardized but there is no common standard for the precise timings of all the procedures.No more than 7 studies focusing on the influence of ICSI procedure timings on the outcoming results were published [1–8], with discrepancies in the conclusions.Text is available under Creative Commons Attribution-Share Alike (CC-BY-SA) and/or GNU Free Documentation License (GFDL). This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. To analyze the impact of oocyte denudation and microinjection timings on intracytoplasmic sperm injection (ICSI) outcomes. We included ICSI cycles with the following parameters: rank 1 or 2, female age Intracytoplasmic sperm injection (ICSI) is the treatment of choice for couples with severe male infertility.